The NuGenia Integrity Protocol

NuGenia Logics™

At NuGenia Research™, every compound released undergoes a structured, multi-stage analytical validation process designed to confirm molecular identity, purity, and batch traceability. We do not simply synthesize peptides. We engineer, verify, and document structural integrity.

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01 — Sequence Architecture & Molecular Blueprint

Every compound begins with defined amino acid architecture. Sequence parameters are established prior to synthesis using structural alignment models and theoretical molecular weight projections. This blueprint phase ensures:

• Accurate amino acid sequencing
• Theoretical molecular weight validation
• Structural alignment with intended molecular design

Precision begins before synthesis — not after.

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02 — Controlled Solid-Phase Peptide Synthesis (SPPS)

Compounds are synthesized using automated solid-phase peptide synthesis systems (SPPS). Amino acids are sequentially coupled under controlled reaction conditions to maximize coupling efficiency and minimize sequence truncation. Each synthesis cycle is monitored to support reproducibility and structural consistency. Precision is not assumed — it is measured.

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03 — Cleavage & High-Performance Liquid Chromatography (HPLC) Purification

Following synthesis, peptides undergo cleavage from resin supports and purification via High-Performance Liquid Chromatography (HPLC). This stage isolates the target compound from:

• Truncated sequences
• Residual reagents
• Synthesis byproducts

Purity thresholds are validated prior to batch progression. Target analytical purity: ≥99% HPLC

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04 — Molecular Identity Confirmation via Mass Spectrometry

Each production lot undergoes mass spectrometry (MS) analysis to confirm:

• Molecular weight accuracy
• Structural integrity
• Sequence alignment consistency

Identity confirmation ensures that the compound released matches its intended molecular design. No assumptions. Only verification.

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05 — Lyophilization & Stability Control

Validated compounds are freeze-dried (lyophilized) into stable powder form to preserve molecular integrity during storage and transport. Environmental handling protocols are maintained to reduce degradation risk and ensure compound stability. Stability preservation supports research reliability.

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06 — Analytical Release & Batch Traceability

Prior to release, every lot is:

• Assigned a unique batch number
• Accompanied by a Certificate of Analysis (CoA)
• Verified for purity and molecular identity
• Documented for full traceability

Each vial is traceable to its originating batch and analytical documentation. Structured documentation supports reproducibility and research continuity.

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