Calculate your exact peptide reconstitution volume and syringe draw in seconds. New to peptides? Use the quick-start guide to auto-fill common protocols.
40+ peptide presets · 14 pathwaysU100 / U50 / 1 mL syringe supportShareable calculation linksResearch use only
Optional — Peptide Quick-Start Guide
New to peptides? Select yours below for a suggested dose range and auto-fill. Skip this step if you already know your protocol.
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⚠ Research Use Only. This calculator is for educational and research purposes only. NuGenia Logics™ does not provide medical advice, clinical guidance, or dosing protocols. The information provided by this tool is not intended to diagnose, treat, cure, or prevent any disease or health condition. Always consult a licensed healthcare professional before handling or administering any research compound.
How to use this calculator
Four steps to your exact injection volume.
1
Enter vial strengthThe total milligrams (mg) of lyophilized peptide in your vial. Common vial sizes are 2, 5, and 10 mg.
2
Add water volumeThe amount of bacteriostatic water (BAC water) you will inject into the vial to reconstitute. More water = lower concentration.
3
Set your doseThe micrograms (mcg) you intend to inject per session, based on your research protocol or researcher's guidance.
4
Read the resultThe calculator shows your injection volume in mL and the exact unit mark to draw to on your insulin syringe.
How to calculate peptide dosage
Peptide dosage calculation requires three pieces of information: the total amount of peptide in your vial (in milligrams), the volume of bacteriostatic water used for reconstitution (in milliliters), and your desired dose per injection (in micrograms).
The formula is straightforward:
Solution concentration (mcg/mL) = Total peptide (mg) × 1000 ÷ Water volume (mL)
With a 5 mg vial reconstituted in 2 mL of bacteriostatic water, your concentration is 2,500 mcg/mL. For a 250 mcg dose, you inject 0.10 mL — or draw to the 10-unit mark on a U100 insulin syringe.
Why the syringe type matters
A U100 insulin syringe holds 1 mL total across 100 unit marks, so each unit = 0.01 mL. A U50 syringe holds 0.5 mL across 50 units — the same physical scale, but the math differs. Always verify which syringe you are using before drawing your dose.
Bacteriostatic water vs. sterile water
Bacteriostatic water (BAC water) contains 0.9% benzyl alcohol as a preservative, extending the shelf life of your reconstituted peptide solution to approximately 28 days when refrigerated. Plain sterile water should only be used for single-use applications, as it contains no preservative to inhibit bacterial contamination after the vial seal is broken.
Peptide calculator FAQ
Common questions about peptide dosage, reconstitution, and injection technique.
How do you calculate peptide dosage?
Peptide dosage is calculated by dividing your desired dose (in mcg) by the concentration of your reconstituted solution (mcg/mL). Concentration is calculated by dividing the total peptide content of the vial (in mcg) by the volume of bacteriostatic water added (in mL). For example: a 5 mg vial reconstituted in 2 mL of BAC water has a concentration of 2,500 mcg/mL. For a 250 mcg dose: 250 ÷ 2,500 = 0.10 mL per injection.
How much bacteriostatic water do I use to reconstitute a peptide vial?
Most research protocols use 1–2 mL of bacteriostatic water per vial. Using less water produces a more concentrated solution, meaning you inject a smaller volume per dose. Using more water creates a more dilute solution, which can make it easier to measure very small doses accurately. For most 5 mg vials, 2 mL is a practical starting point.
What syringe should I use for subcutaneous peptide injections?
A U100 insulin syringe (100 units = 1 mL) is the most commonly used syringe for subcutaneous peptide injections. Each unit mark on the scale equals exactly 0.01 mL, making it very precise for small volumes. Needles in the 28–31 gauge, 0.5-inch range are typically preferred for subcutaneous administration. This calculator converts your required draw volume directly into U100 unit marks.
What is the difference between mg and mcg in peptide dosing?
1 mg (milligram) equals 1,000 mcg (micrograms). Research peptide vials are typically sold by total content in milligrams (e.g., 5 mg per vial), while research protocols often express individual doses in micrograms (e.g., 250 mcg per injection). This is one of the most common sources of dosing confusion — this calculator handles the unit conversion automatically so you don't need to think about it.
What is bacteriostatic water and why is it used for peptides?
Bacteriostatic water (BAC water) is sterile water for injection that contains 0.9% benzyl alcohol as a preservative. The benzyl alcohol inhibits bacterial growth in the vial after the rubber stopper has been punctured, extending the shelf life of the reconstituted peptide solution to approximately 28 days when stored refrigerated (2–8°C). Plain sterile water offers no such protection and should only be used for single-dose applications.
How should I store reconstituted peptides?
Reconstituted peptide solutions should be stored refrigerated at 2–8°C (36–46°F) and are typically stable for 28–30 days with bacteriostatic water. Avoid freezing the reconstituted solution and protect it from direct light. Lyophilized (freeze-dried, unreconstituted) peptide powder can typically be stored at -20°C for extended periods — often years — before reconstitution.
What is a U100 insulin syringe in mL?
A U100 insulin syringe holds 1 mL total volume across 100 unit marks. Each unit equals exactly 0.01 mL. So 10 units = 0.10 mL, 50 units = 0.50 mL, and 100 units = 1.00 mL. This makes the U100 syringe ideal for measuring small peptide injection volumes with precision.
